LOCUS       pGB-11-12-ccdB      3470 bp    DNA     circular UNA 2-DEC-2020
DEFINITION  LMBP 12035.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Accession number: LMBP 12035
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 2-DEC-2020
FEATURES             Location/Qualifiers
     misc_feature    1..224
                     /label=Tn7\recombination\sequence
     CDS             complement(291..824)
                     /label=gentamycin\resistance
     promoter        891..925
                     /label=intl1\promoter
     promoter        complement(1013..1041)
                     /label=PcS\promoter
     misc_feature    1112..1145
                     /label=loxP
     misc_feature    1183..1186
                     /label=GoldenBac\overhang\11
     promoter        1237..1325
                     /label=AcNPV-PH\promoter
     misc_feature    1396..1479
                     /label=Twin-Strep-tag
     misc_feature    1489..1512
                     /label=PreScission\site
     CDS             1638..1946
                     /label=ccdB
     misc_feature    1966..1983
                     /label=His-tag
     terminator      complement(2105..2201)
                     /label=SV40\polyA\late
     terminator      2105..2201
                     /label=SV40\polyA\early
     misc_feature    2382..2385
                     /label=GoldenBac\overhang\12
     misc_feature    2393..2558
                     /label=Tn7\recombination\sequence
     rep_origin      3118..3421
                     /label=pMB1\ori
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg taaagactat attcgcggcg ttgtgacaat
      241 ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg
      301 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga
      361 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg
      421 ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg
      481 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga
      541 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat
      601 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg
      661 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg
      721 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta
      781 gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa
      841 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
      901 tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
      961 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg
     1021 aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca
     1081 cccggcaacc ttgggcagca gcgaagtcgc cataacttcg tatagcatac attatacgaa
     1141 gttatctgta actataacgg tcctaaggta gcgaaggtct caagaagttt aaacactagt
     1201 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     1261 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     1321 taaatattcc ggattattca taccgtccca ccatcgggcg acggatcccg gtccgaaacc
     1381 atgaaaggga gcgcttggag ccacccgcag ttcgaaaaag gtggaggttc tggcggtgga
     1441 tcgggaggtt cagcgtggag ccacccgcag ttcgagaaaa ccgcgggtct ggaagttctg
     1501 ttccaggggc ccatggacta atgaggtacc ggatccgaat tcgagctccg tcgacaagct
     1561 tgatatcgaa ttcctgcagc ccaaactgca gttgacaaca taaaaacttt gtgttatact
     1621 tgtaacgtaa ggaggtaatg attcagttta aggtttacac ctataaaaga gagagccgtt
     1681 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     1741 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     1801 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     1861 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     1921 ttaacctgat gttctgggga atataagctt gcggccgcac tcgagcacca ccaccaccac
     1981 cactgacatg cggtaccaag cttgtcgaga agtactagag gatcataatc agccatacca
     2041 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2101 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2221 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta
     2281 gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     2341 caataactat cataacccct agggtatacc catctaattg ggttgtgaga ccaaccagat
     2401 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
     2461 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
     2521 ccacccctcc cagttcccaa ctattttgtc cgcccacaac cggttgactt gggtcaactg
     2581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2881 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3421 tgacttgggt cgctcttcct gtggatgcgc agatgccctg cgtaagcggg
//
